Part:BBa_K1361000
Curli Fiber generator under Pbad induction with positive regulation
We assembled this part in order to examine the concept that change of cell motility by control of CheZ chemotaxis regulator could disturb pre-expressed curli fiber structure. If the inducer occurs, in this case_acacia, CsgB protein will secrete into extracellular and act as nucleator to recruit soluble CsgA monomer into amyloid fibres, and the transcription of CheZ will be downgrade via cI-Plamda NOT gate. If induction condition disappears at a certain point, suppression of CheZ will be discharged. As the result, cells will be more moveable thus disturb or inhibit the establishment of curli structure. And in our final device, the modified CsgA that has ability to absorb nanogold, thus the dynamic detection could achieve by monitoring current change between two electrodes in the culture.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 1545
Illegal NheI site found at 1568 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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